"In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. The use of thermodynamics in biological research is used as an “energy book-keeping system. While the str ..."
"Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections in Two-Component Signaling Systems, Part B include: Structural ApproachesReconstitution of Heterogeneous SystemsIntracellular Methods and AssaysGenome-Wide Analyses of Two-Component SystemsPresents detail ..."
"This book provides a single source of information for all molecular methods. Expert advice is given on sample collection and storage for molecular work."
"To understand the structure and dynamics of DNA and its interactions with ligands, a complete analysis of its confirmational flexibility is required. This volume and its companion, Volume 211 of Methods of Enzymology, collate in one source all the methods and approaches needed to achieve this goal."
"This volume provides, for the first time, an extensive compilation of those state-of-the-art methods that have revolutionized the use of mice as an experimental system. This volume is a unique source for detailed descriptions to be used in research by both experienced and novice researchers - Germ cells and embryos; Fertilization; Gene expression: messenger RNA, reporter genes, proteins, methylation; Gene identification; Nuclear transpl ..."
"The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication ..."
"This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs. The critically acclaimed laboratory ..."
"The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 270 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publicat ..."
"The Cumulative Subject Index for Volumes 195(198 and 200(227 includes the contents of the volumes covered, the subject index, contributor index, and a complete list of all the volumes published in the series. This volume supplements the Methods in Enzymology Cumulative Index Volumes 33, 75, 95, 120, 140, 175, and 199."
Biothiols, Part B(1st Edition) Glutathione and Thioredoxin: Thiols in Signal Transduction and Gene Regulation, Volume 252 (Methods in Enzymology) by Helmut Sies, MelvinI. Simon, John N. Abelson, Lester Packer, L. Packer Hardcover, 382 Pages, Published 1995 by Academic Press ISBN-13: 978-0-12-182153-1, ISBN: 0-12-182153-6
"The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for res ..."
"[32] Classification of Penicillium Fungi by Cross-Partition Analysis By Goran
Blomquist and Gunnar B. Strom Introduction Penicillium species are normally
classified according to morphological criteria. This has caused confusion among
mycologists since certain species, especially within the P. viridicatum ... 1 G.
Blomquist, B. Andersson, K. Anderson, and I. Brondz, J. Microbiol. Methods, in
press (1992). 2 A. C. M. Weijman, in "Ana ..."
"The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. ..."
"The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences."
"This volume provides up-to-date information on the purification and characterization of the enzymes involved in phospholipid biosynthesis and on the mechanisms utilized by cells for the transfer of phospholipids. It also includes protocols for generating phospholipid biosynthetic mutants."
"The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences."
"This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases. Key Features* Adenylyl cyclase: determination of activity* Preparation of materials useful in purification of components of hormonally responsive cyclase systems* Purification guanine nucleotide-dependent regulato ..."
Retinoids, Part B(1st Edition) Cell Differentiation and Clinical Applications, Volume 190: Volume 190: Retenoids Part B (Methods in Enzymology) by John N. Abelson, MelvinI. Simon, Helmut Sies, Lester Packer, L. Packer Hardcover, 488 Pages, Published 1991 by Academic Press ISBN-13: 978-0-12-182091-6, ISBN: 0-12-182091-2
"[29] Antioxidant Activity of Retinoids By Midori Hiramatsu and Lester Packer
Introduction Although the effects of retinoids are numerous in biological systems,
among them are indications that retinoids may exert some of their actions by
virtue of ..."
"This book provides up-to-date, state-of-the-art methods for the purification of metallothionein and related proteins, for the study of metal binding, for the quantitation of metallothionein, and for the study of the physical and biological properties of metallothionein. New views of the structure and function of metallothionein are also included in some chapters."